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Volume 2 No. 1, 2012
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+ Volume 2, 2012
Issue 1
+ Volume 1, 2011
Insight Bioinformatics
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Volume 2 Issue 1, 2012
In silico
Analyses of Flavin Reductase from
Citrobacter freundii
A1
Giek Far Chan
,
Abdull Rahim Mohd Yusoff
and
Noor Aini Abdul Rashid
Abstract:
Objective:
The aim of the present study was to describe the structural and phylogenetic features of flavin reductase from
C. freundii
A1 using bioinformatics tools.
Materials and methods:
The flavin reductase (
fre
) gene from a dye-degrading bacterium,
C. freundii
A1 was isolated and amplified by Polymerase Chain Reaction (PCR). The gene encodes for NAD(P)H:flavin oxidoreductase, an enzyme that catalyzes the reduction of soluble flavins by reduced pyridine nucleotides this was believed to be the azoreductase from
C. freundii
A1. The gene, approximately 0.8 kb was sequenced and
in silico
analyses of the nucleotide sequence were performed.
Results:
From phlyogenetic analyses, we observed that flavin reductase enzyme generally existed in most microorganisms and the enzyme from
C. freundii
A1 is conserved among the Gram-negative bacteria. The protein function of the flavin reductase coded was predicted based on the motifs of deduced amino acid sequence. The amino acid sequence of flavin reductase from
C. freundii
A1 was compared with other azoreductases and was found to be a unique NADPH-preferred azoreductase.
Conclusion:
Hence,
in silico
characterization of flavin reductase gene presented various features of the gene and this would facilitate molecular studies of the gene and reveal the functional role of the enzyme.
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How to Cite:
Giek Far Chan
,
Abdull Rahim Mohd Yusoff
and
Noor Aini Abdul Rashid , 2012.
In silico
Analyses of Flavin Reductase from
Citrobacter freundii
A1. Insight Bioinformatics, 2: 1-8
DOI:
10.5567/BIOINFO-IK.2012.1.8
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