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Insight Biotechnology>>  Volume 1 Issue 1, 2011
Alteration of Protein Patterns in Callus Cultures of Citrullus colocynthis in Relation to Plant Growth Regulators
Amal A. Mohamed , Farouk K. El-Baz , Sami I. Ali , Mahmoud M. Saker and Ahmad K. Hegazy
 
Abstract: Callus and cell culture could not only be an alternate continue source of proteins, but could also be a useful and important model system to study their regulation and biosynthesis so, the present study aimed to investigate the effect of different combinations of 2,4 dichloro phenoxy acetic acid (2,4-D) with kinetin (kin) and Benzyl Adenine (BA) with α-Naphthalene Acetic Acid (NAA) on: (1) the percentage of callus frequency and (2) protein profiles of Citrullus colocynthis stems, leaves and roots derived callus cultures. The present results reported that the highest percentage of callus frequency (98.9%) was obtained from stem callus grown on MS media supplemented with (1.0 mg L-1) 2,4-D + (1.0 mg L-1) kin. Sodium Dodecyl Sulfate (SDS) Polyacrylamide Gel Electrophoresis (PAGE) analysis of total soluble protein revealed expression of new protein band (65 kDa) only in stem derived callus cultured on MS media supplemented with (6.0 mg L-1) 2,4-D + (2.0 mg L-1) kin. Leaf derived callus cultured on MS media supplemented with (2.0 mg L-1) 2,4-D + (1.0 mg L-1) kin showed new protein band with molecular weight 112 kDa. Moreover, the root derived callus revealed expression of new protein band (82 kDa) with MS media supplemented with (6.0 mg L-1) 2,4-D + (2.0 mg L-1) kin. However, MS media supplemented with (0.01 mg L-1) BA + (1.0 mg L-1) NAA exhibit new protein band (72 kDa) in the root derived callus. The present results concluded that changes in the protein pattern appear to correlate with colocynth callus percentage and different combinations of plant growth regulators.
 
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    How to Cite:
Amal A. Mohamed, Farouk K. El-Baz, Sami I. Ali, Mahmoud M. Saker and Ahmad K. Hegazy , 2011. Alteration of Protein Patterns in Callus Cultures of Citrullus colocynthis in Relation to Plant Growth Regulators. Insight Biotechnology, 1: 1-6
DOI: 10.5567/IBIOT-IK.2011.1.6
 
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